Browsing by Author "Betty Magambo"

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Inhibition Of Cell Death As An Approach For Development Of Transgenic Resistance Against Fusarium Wilt Disease
(Afr. J. Biotechnol., 2016-04-17) Betty Magambo; Khanna Harjeet; Geofrey Arinaitwe; Sali Tendo; Ivan Kabiita Arinaitwe; Jerome Kubiriba; Wilberforce Tushemereirwe; James Dale
Fusarium oxysporum f. sp. cubense (Foc) is one of the major threats to dessert banana (Musa spp.) production. In Uganda, ‘Sukali Ndiizi’ is one of the most popular dessert banana cultivars and it is highly susceptible to Fusarium wilt. Development of resistant cultivars through transgenic approaches has shown to offer one of the most effective control options for most diseases. The transgenic approaches for providing plant disease resistance have mainly been through either enzymatic destruction of pathogen structures, neutralization of pathogen and its products or production of metabolites that eventually kill the pathogen. However in recent years, methods that prevent cell death of host plant after infection especially for necrotrophic pathogens like F. oxysporum have registered success in providing resistance in several crops. We investigated whether the transgenic expression of a programmed cell death inhibition gene in Sukali Ndiizi could be used to confer Fusarium resistance to Foc race 1. Embryogenic cell suspensions of cv. ‘Sukali Ndiizi, were stably transformed with a synthetic, plant-codon optimise mCed-9 gene. Twenty-eight independently transformed plant lines were regenerated. The lines were inoculated with Foc race 1 and observed for 13 weeks in small-plant glasshouse. Three transgenic lines showed significantly lower internal and external disease symptoms than the wild-type susceptible ‘Sukali Ndiizi’ banana plants used as controls. This is the first report from Africa on the generation of Fusarium wilt tolerant transgenic ‘Sukali Ndiizi’, a very popular but rapidly diminishing African dessert banana.
Viability of Deficit Irrigation Pre-Exposure in Adapting Robusta Coffee to Drought Stress
(Agronomy, 2023-02-25) Godfrey Sseremba; Pangirayi Bernard Tongoona; Pascal Musoli; John Saviour Yaw Eleblu; Leander Dede Melomey; Daphne Nyachaki Bitalo; Evans Atwijukire; Joseph Mulindwa; Naome Aryatwijuka; Edgar Muhumuza; Judith Kobusinge; Betty Magambo; Godfrey Hubby Kagezi; Eric Yirenkyi Danquah; Elizabeth Balyejusa Kizito; Gerald Kyalo; Emmanuel Iyamulemye; Geofrey Arinaitwe
Coffea canephora has high but inadequately exploited genetic diversity. This diversity, if well exploited, can sustain coffee productivity amidst climate change effects. Drought and heat stress are major global threats to coffee productivity, quality, and tradable volumes. It is not well understood if there is a selectable variation for drought stress tolerance in Robusta coffee half-sibs as a result of watering deficit pre-exposure at the germination stage. Half-sib seeds from selected commercial clones (KR5, KR6, KR7) and a pipeline clone X1 were primed with deficit watering at two growth stages followed by recovery and later evaluated for tolerance to watering deficit stress in three different temperature environments by estimation of plant growth and wilt parameters. Overall, the KR7 family performed the best in terms of the number of individuals excelling for tolerance to deficit watering. In order of decreasing tolerance, the 10 most promising individuals for drought and heat tolerance were identified as: 14.KR7.2, 25.X1.1, 35.KR5.5, 36.KR5.6, 41.KR7.5, 46.KR6.4, 47.KR6.5, 291.X1.3, 318.X1.3, and 15.KR7.3. This is the first prospect into the potential of C. canephora half-sibs’ diversity as an unbound source of genetic variation for abiotic stress tolerance breeding.