Browsing by Author "Hillary Agaba"

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    Morphological Characteristics and Growth Performance of Bambusa vulgaris Schrad. Ex J. C. Wendl in Selected Agro-Ecologies of Uganda
    (East African Journal of Forestry & Agroforestry, 2024-03-08) Fred Kalanzi; Isaac Kiyingi; Christine Kalembe Mwanja; Hillary Agaba; Michael Malinga; Selim Reza; Durai Jayaraman
    Bamboo is a versatile and valuable resource that has been shown to have social, ecological, and economic benefits in many countries worldwide. Even though Uganda has a suitable environment for bamboo growth, it remains underutilized. One of the key challenges in promoting bamboo farming is the need for more information about the growth performance of different bamboo species in various site conditions. Therefore, this study determined the morphological characteristics and growth performance of Bambusa vulgaris in Uganda. A total of 24 bamboo farms were selected from a list of bamboo farmers provided by key informants using stratified sampling. These farms had relatively uniform B. vulgaris stands and were drawn from Uganda's three agro-ecological zones (AEZs): the Southwestern, West Nile, and Lake Victoria crescent agro-ecological zones. Eight temporary sample plots were established in each zone, and various growth parameters were measured. One-way Analysis of Variance (ANOVA) followed by Sidak post-hoc test was used to compare the performance of B. vulgaris across the different agro- ecological zones based on growth parameters. The results of the study showed that the total above-ground biomass was highest in the Lake Victoria crescent (24.773 kg/ha), followed by the West Nile (17.141 kg/ha) and the Southwestern (15.125 kg/ha) agro-ecological zones. The growth rate of B. vulgaris after three years was found to be similar to the outstanding growth rate mentioned in the literature. The growth performance of B. vulgaris was notably better in the L. Victoria crescent compared to the West Nile and Southwestern highlands – suggesting that efforts to promote the growing of B. vulgaris should prioritize the L. Victoria crescent agro-ecological zone. Overall, this study provides valuable information on the growth performance of B. vulgaris in different agro-ecological zones in Uganda. This information can be used to guide the promotion and development of bamboo farming in the country, which has the potential to improve livelihoods and contribute to sustainable development.
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    SilicoDArT and SNP markers for genetic diversity and population structure analysis of Trema orientalis; a fodder species
    (PLOS One, 2022-08-22) Judith Ssali Nantongo; Juventine Boaz Odoi; Hillary Agaba; Samson Gwali
    Establishing the genetic diversity and population structure of a species can guide the selection of appropriate conservation and sustainable utilization strategies. Next-generation sequencing (NGS) approaches are increasingly being used to generate multi-locus data for genetic structure determination. This study presents the genetic structure of a fodder species -Trema orientalis based on two genome-wide high-throughput diversity array technology (DArT) markers; silicoDArT and single nucleotide polymorphisms (SNPs). Genotyping of 119 individuals generated 40,650 silicoDArT and 4767 SNP markers. Both marker types had a high average scoring reproducibility (>99%). Genetic relationships explored by principal coordinates analysis (PCoA) showed that the first principal coordinate axis explained most of the variation in both the SilicoDArT (34.2%) and SNP (89.6%) marker data. The average polymorphic information content did not highly differ between silicoDArT (0.22) and SNPs (0.17) suggesting minimal differences in informativeness in the two groups of markers. The, mean observed (Ho) and expected (He) heterozygosity were low and differed between the silicoDArT and SNPs respectively, estimated at Ho = 0.08 and He = 0.05 for silicoDArT and Ho = 0.23 and He = 0.19 for SNPs. The population of T. orientalis was moder- ately differentiated (FST = 0.20–0.53) and formed 2 distinct clusters based on maximum likelihood and principal coordinates analysis. Analysis of molecular variance revealed that clusters contributed more to the variation (46.3–60.8%) than individuals (32.9–31.2%). Overall, the results suggest a high relatedness of the individuals sampled and a threatened genetic potential of T. orientalis in the wild. Therefore, genetic management activities such as ex-situ germplasm management are required for the sustainability of the species. Ex-situ conservation efforts should involve core collection of individuals from different populations to capture efficient diversity. This study demonstrates the importance of silicoDArT and SNP makers in population structure and genetic diversity analysis of Trema orientalis, useful for future genome wide studies in the species.