Browsing by Author "Narayanan N. Narayanan"

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    Mutations in DNA polymerase δ subunit 1 co- segregate with CMD2-type resistance to Cassava Mosaic Geminiviruses
    (Nature Communications, 2022-07-07) Yi-Wen Lim; Ben N. Mansfeld; Pascal Schläpfer; Kerrigan B. Gilbert; Narayanan N. Narayanan; Weihong Qi; Qi Wang; Zhenhui Zhong; Adam Boyher; Jackson Gehan; Getu Beyene; Zuh-Jyh Daniel Lin; Williams Esuma; Suhua Feng; Christelle Chanez; Nadine Eggenberger; Gerald Adiga; Titus Alicai; Steven E. Jacobsen; Nigel J. Taylor; Wilhelm Gruissem; Rebecca S. Bart
    Cassava mosaic disease (CMD) suppresses cassava yields across the tropics. The dominant CMD2 locus confers resistance to cassava mosaic geminiviruses. It has been reported that CMD2-type landraces lose resistance after regeneration through de novo morphogenesis. As full genome bisulfite sequencing failed to uncover an epigenetic mechanism for this loss of resistance, whole genome sequencing and genetic variant analysis was performed and the CMD2 locus was fine-mapped to a 190 kilobase interval. Collectively, these data indicate that CMD2-type resistance is caused by a nonsynonymous, single nucleotide polymorphism in DNA polymerase δ subunit 1 (MePOLD1) located within this region. Virus-induced gene silencing of MePOLD1 in a CMD-susceptible cassava variety produced a recovery phenotype typical of CMD2-type resistance. Analysis of other CMD2-type cassava varieties identified additional candidate resistance alleles within MePOLD1. Genetic variation of MePOLD1, therefore, could represent an important genetic resource for resistance breeding and/or genome editing, and elucidating mechanisms of resistance to geminiviruses.