Browsing by Author "S. M. Magambo"

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    Developing a cell suspension system for Musa-AAA-EA cv. ‘Nakyetengu’: a critical step for genetic improvement of Matooke East African Highland bananas
    (In Vitro Cellular & Developmental Biology - Plant, 2014-04-26) Priver Namanya; G. Mutumba; S. M. Magambo; W. Tushemereirwe
    Embryogenic cell suspensions of triploid East African Highland bananas (Musa AAA-EA) were initiated and generated using cooking cultivar ‘Nakyetengu’ belonging to the Nakabululu clone set. Immature male flowers produced embryogenic calli consisting of embryos and friable tissue after 4 mo culture on a modified MA1 callus induction medium. Friable calli were initiated and maintained in liquid MA2 medium. A cell growth rate of 1.5–2.0 sedimented cell volume (SCV) per month was observed. Embryo development was observed at 2.18 × 103 embryos per mL SCV. Germination of these embryos was observed at 2.8% and 6.2% for two cell suspension lines. Plant regeneration efficiency was 60–100%, all producing normal plants with a shoot and roots at weaning. In the field, somatic cell-derived plants were all normal morphology and comparable to control plants during vegetative and reproductive stages. This study is a breakthrough for recalcitrant East African Highland banana and offers a system that can provide essential raw materials for associated germ- plasm improvement through genetic engineering approaches.
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    Developing A Cell Suspension System For Musa-Aaa-Ea Cv. ‘Nakyetengu’: A Critical Step For Genetic Improvement Of Matooke East African Highland Bananas
    (Vitro Cellular & Developmental Biology - Plant, 2014-04-18) Priver Namanya; G. Mutumba; S. M. Magambo; W. Tushemereirwe
    Embryogenic cell suspensions of triploid East African Highland bananas (Musa AAA-EA) were initiated and generated using cooking cultivar ‘Nakyetengu’ belonging to the Nakabululu clone set. Immature male flowers produced embryogenic calli consisting of embryos and friable tissue after 4 mo culture on a modified MA1 callus induction medium. Friable calli were initiated and maintained in liquid MA2 medium. A cell growth rate of 1.5–2.0 sedimented cell volume (SCV) per month was observed. Embryo development was observed at 2.18 × 103 embryos per mL SCV. Germination of these embryos was observed at 2.8% and 6.2% for two cell suspension lines. Plant regeneration efficiency was 60–100%, all producing normal plants with a shoot and roots at weaning. In the field, somatic cell-derived plants were all normal morphology and comparable to control plants during vegetative and reproductive stages. This study is a breakthrough for recalcitrant East African Highland banana and offers a system that can provide essential raw materials for associated germ- plasm improvement through genetic engineering approaches.